FastQCFastQC Report
Fri 10 Feb 2017
ERR1147565.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147565.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3607650
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271210.7517636134325669No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154750.4289495932255069No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130310.36120466231480325No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43760.12129779773536789No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA36400.10089670561168629No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98450.057.8685461
GTATCAA194100.057.7218781
AACGCAG257200.043.7380946
TCAACGC261400.042.785594
ATCAACG262000.042.6887933
CAACGCA267350.041.899555
TATCAAC273950.040.9792442
ACGCAGA301750.037.3569227
CGCAGAG305950.036.9213568
GCAGAGT326200.034.6658069
GTACATG305100.034.1657031
TACATGG311550.033.4174772
ACATGGG311650.033.02453
CATGGGT118100.032.6918954
CATGGGG139600.031.108764
ATGGGTG61900.029.9849415
ATGGGGG61400.028.9695745
AGAGTAC319600.028.25323310-11
AGTACTT151750.026.5180912-13
GAGTACT147350.026.42181412-13
Produced by FastQC (version 0.11.5)