FastQCFastQC Report
Fri 10 Feb 2017
ERR1147564.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147564.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2688824
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180350.6707393269325177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109280.4064230310351291No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88100.32765253508597064No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31230.11614743099585542No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT27720.10309339696462096No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA155850.050.2266241
GGTATCA88550.048.6704141
AACGCAG190550.040.674986
ATCAACG202400.038.3824423
TCAACGC202950.038.2777184
CAACGCA207500.037.3237085
TATCAAC211500.036.9004332
ACGCAGA221150.035.0737767
CGCAGAG224400.034.644688
CATGGGT69000.033.7931634
GTACATG233250.033.2280121
GCAGAGT237900.032.753729
ACATGGG233450.032.5639423
TACATGG238700.032.4464042
CATGGGG113950.031.1639044
ATGGGTG38850.030.774925
ATGGGGG53750.029.5477685
GCGTAAT1055.891987E-428.3462581
AGAGTAC236800.026.4879710-11
GTAAGTA19600.025.4936052
Produced by FastQC (version 0.11.5)