FastQCFastQC Report
Fri 10 Feb 2017
ERR1147563.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147563.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2373110
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129450.545486724172078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76240.3212661865653088No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60550.2551504144350662No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23850.10050103029358098No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA127750.047.2985731
GGTATCA76400.040.9859771
AACGCAG159150.038.0110056
ATCAACG163250.037.0222663
TCAACGC163250.036.9842724
CAACGCA166050.036.2523965
TATCAAC172350.035.136542
ACGCAGA182200.033.1682177
CGCAGAG184500.032.7869768
GCAGAGT199250.030.4792429
GTACATG186650.029.2790931
TACATGG188350.028.9618452
ACATGGG187950.028.4854243
CATGGGG77300.028.1647454
CATGGGT35300.026.7933254
ATGGGGG38750.026.5564165
GCGTATC1608.711213E-626.0447981
AGAGTAC192000.024.76817310-11
GAGTACT81500.024.7410912-13
AGTACTT85600.024.07721312-13
Produced by FastQC (version 0.11.5)