FastQCFastQC Report
Fri 10 Feb 2017
ERR1147562.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147562.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2987316
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164550.5508289046086855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95400.3193502126992926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74950.25089411364582787No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30650.10260046141754003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68050.054.4910471
GTATCAA127350.053.46781
TCAACGC166950.040.224364
AACGCAG166500.040.1544536
ATCAACG169600.039.5640953
TATCAAC172750.039.2558862
CAACGCA171250.039.110155
ACGCAGA193600.034.501787
CGCAGAG198400.033.697048
GCAGAGT212050.031.4718079
GTACATG201300.030.809821
TACATGG204900.030.0480562
ACATGGG204800.029.3075263
CATGGGG94050.027.5113644
CATGGGT45700.027.4629974
AGAGTAC205850.025.9184310-11
CAGAGTA211250.024.69278110-11
GAGTACT92150.024.39852312-13
AGTACTT97250.023.0884312-13
ATGGGGG42250.022.384745
Produced by FastQC (version 0.11.5)