FastQCFastQC Report
Fri 10 Feb 2017
ERR1147561.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147561.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3928416
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131760.335402360646123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77880.1982478434055864No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62010.15784988147894724No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA109600.056.591781
GGTATCA54850.052.090781
TCAACGC143000.042.6763084
ATCAACG145300.042.0433123
CAACGCA145500.041.7795145
AACGCAG145550.041.765166
TATCAAC162700.037.8760152
ACGCAGA172050.035.2972647
CGCAGAG179600.033.8796778
GTACATG196300.031.5665171
GCAGAGT195900.031.1821449
TACATGG203050.030.6129652
ACATGGG202850.029.9100573
CATGGGG111600.028.1946684
AGAGTAC183650.026.15300410-11
ATGGGGG56850.024.064285
CAGAGTA192200.023.39582410-11
GAGTACT80500.022.01863312-13
AGTACTT85700.022.00130312-13
CATGGGT48700.021.2518024
Produced by FastQC (version 0.11.5)