FastQCFastQC Report
Fri 10 Feb 2017
ERR1147560.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147560.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2704988
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173420.6411119014206348No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96780.3577834726069025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84240.31142467175455124No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30690.11345706524391237No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA128200.049.967181
GGTATCA73400.044.0417251
AACGCAG156650.040.5598376
TCAACGC170000.037.5503654
ATCAACG170150.037.483693
CAACGCA173250.036.7766045
TATCAAC182550.035.1005022
ACGCAGA184350.034.3983277
CGCAGAG190150.033.3491028
GTACATG186500.032.910951
TACATGG192150.031.8606382
ACATGGG190750.031.7513873
CATGGGG99900.031.7413314
GCAGAGT203150.031.332159
ATGGGGG43200.028.3686585
CATGGGT37250.027.9495724
AGAGTAC196200.026.72677810-11
TAACGCA2401.3053068E-824.788614
AGTACTT97400.024.67434912-13
CAGAGTA200900.023.99917610-11
Produced by FastQC (version 0.11.5)