FastQCFastQC Report
Fri 10 Feb 2017
ERR1147559.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147559.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2456035
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156630.6377352114281759No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99840.40650886489809795No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75870.3089125358555558No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27460.11180622425983344No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67450.051.8143771
GTATCAA118950.050.9038471
AACGCAG146500.040.5657276
TCAACGC155450.038.4597784
ATCAACG155650.038.2972643
CAACGCA158100.037.514125
TATCAAC168000.035.8360672
ACGCAGA170650.034.894687
CGCAGAG172850.034.4498448
GCAGAGT185400.032.2141469
GTACATG157950.030.456911
TACATGG163600.029.3453542
ACATGGG162450.029.1868823
AGAGTAC180350.027.62429810-11
CATGGGT42000.026.7697264
CGTATAC1358.2587074E-526.4403133
CATGGGG84450.026.3453144
CAGAGTA185450.025.53341110-11
ATGGGTA21450.025.514795
GAGTACT90750.025.20416512-13
Produced by FastQC (version 0.11.5)