FastQCFastQC Report
Fri 10 Feb 2017
ERR1147558.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147558.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4234665
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212510.5018342655204131No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120930.28557158594599574No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98870.23347773672769861No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA154750.056.8933641
GGTATCA79400.052.405971
AACGCAG203200.042.7385526
TCAACGC205050.042.3819664
ATCAACG208750.041.6902163
CAACGCA210450.041.2379465
TATCAAC228700.038.2875822
ACGCAGA236550.036.6371887
CGCAGAG244700.035.514188
GCAGAGT262150.033.2409369
GTACATG242150.032.867831
TACATGG249350.031.5383072
ACATGGG248850.031.19533
CATGGGT75300.027.9639784
CATGGGG117250.027.85164
AGAGTAC252150.027.35251610-11
GAGTACT121200.025.59394512-13
CAGAGTA259450.024.92076510-11
AGTACTT127900.024.67177212-13
ATGGGGG51100.024.3285395
Produced by FastQC (version 0.11.5)