FastQCFastQC Report
Fri 10 Feb 2017
ERR1147557.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147557.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4194184
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185340.4418976372996511No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106230.2532793029585731No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84950.2025423777306861No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA149200.055.496871
GGTATCA77400.055.296561
TCAACGC198200.041.4761624
AACGCAG198600.041.3027736
ATCAACG201600.040.866163
CAACGCA203650.040.3369875
TATCAAC219850.037.7448272
ACGCAGA231400.035.3964427
CGCAGAG240650.034.1594778
GCAGAGT260050.031.6568899
GTACATG250550.031.4084991
TACATGG255700.030.8011552
ACATGGG257000.030.2746543
CATGGGG135800.028.9531564
ATGGGGG68000.027.3797595
AGAGTAC246500.026.39911810-11
GAGTACT104600.024.87914312-13
CAGAGTA254750.024.59854310-11
CATGGGT72400.024.1547724
AGTACTT110400.023.59902412-13
Produced by FastQC (version 0.11.5)