FastQCFastQC Report
Fri 10 Feb 2017
ERR1147556.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147556.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2708573
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120580.4451790666155204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64790.23920344771951874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56300.207858529196001No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95350.056.4360471
GGTATCA50200.049.2099881
AACGCAG124500.042.4314466
TCAACGC127100.041.93794
ATCAACG130250.040.9708483
CAACGCA130400.040.6028565
TATCAAC139900.038.3999182
ACGCAGA146600.036.1147167
CGCAGAG151600.034.922958
GCAGAGT164500.032.437459
GTACATG154150.031.8580571
TACATGG158950.030.6163222
ACATGGG159550.030.0910223
CATGGGG90300.028.2627054
AGAGTAC155700.027.94768510-11
ATGGGGG43650.025.2134155
CATGGGT41250.025.0939754
AGTACTT74150.024.86970912-13
CAGAGTA160250.024.35151310-11
GAGTACT71500.023.461912-13
Produced by FastQC (version 0.11.5)