FastQCFastQC Report
Fri 10 Feb 2017
ERR1147555.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147555.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3041765
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163500.5375168693176495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95010.31235154589522857No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75830.24929605015509088No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA120850.051.2338831
GGTATCA68050.049.779981
AACGCAG153400.040.21376
TCAACGC160700.038.497994
ATCAACG162700.038.0266273
CAACGCA164450.037.5477645
TATCAAC173050.036.164812
ACGCAGA182550.033.8557857
CGCAGAG188600.032.768678
GTACATG186200.031.046321
GCAGAGT201950.030.7202939
TACATGG190400.030.4947362
CATGGGG103650.030.2455794
ACATGGG188400.030.218513
AGAGTAC193700.026.20950510-11
ATGGGGG49300.024.9772175
GAGTACT91600.024.57849512-13
CAGAGTA198300.024.22170810-11
AGTACTT94950.023.83661712-13
CATGGGT44550.023.1003724
Produced by FastQC (version 0.11.5)