FastQCFastQC Report
Fri 10 Feb 2017
ERR1147554.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147554.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2375767
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189990.7996996338445648No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112270.4725631764394404No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89130.37516305260574795No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30760.12947397619379342No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28140.11844595871564846No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA146000.053.3638421
GGTATCA80400.047.230761
AACGCAG182300.042.4199456
ATCAACG191050.040.6025283
TCAACGC191900.040.4528354
CAACGCA195900.039.596485
TATCAAC197000.039.557392
ACGCAGA212700.036.440247
CGCAGAG215950.035.836738
GCAGAGT230200.033.7217039
GTACATG198250.031.6137081
TACATGG200600.031.1076452
ACATGGG200200.030.7240873
CATGGGG93450.030.1089254
CATGGGT60800.029.9385834
AGAGTAC223150.027.0832710-11
GAGTACT109900.026.7112212-13
AGTACTT113900.026.66099212-13
ATGGGTG33400.026.1808835
CAGAGTA227400.025.39996110-11
Produced by FastQC (version 0.11.5)