FastQCFastQC Report
Fri 10 Feb 2017
ERR1147553.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147553.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295042
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129881.002901836388318No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77100.595347486799656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59730.46122056273078404No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21410.1653228235068824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA110000.049.3616031
GGTATCA62550.044.3553241
AACGCAG126650.042.408246
TCAACGC140150.038.408134
TATCAAC142350.038.0696532
ATCAACG141800.037.9656073
CAACGCA142900.037.544135
ACGCAGA147350.036.489617
CGCAGAG148250.036.3483288
GCAGAGT156400.034.6063359
GTACATG132400.029.3188381
ATGGGGG21500.028.7715055
TACATGG135200.028.687252
ACATGGG131850.028.6040253
CATGGGG66150.027.78414
TATCACG1306.3887834E-527.4552822
AGAGTAC154950.026.9845210-11
CAGAGTA156900.026.04262510-11
GAGTACT76400.025.92398812-13
AGTACTT78250.025.65313112-13
Produced by FastQC (version 0.11.5)