FastQCFastQC Report
Fri 10 Feb 2017
ERR1147552.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147552.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3575637
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157740.44115216393610424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96370.26951841028605533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76430.21375212304828484No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60300.057.3616141
GTATCAA117900.054.4336551
TCAACGC151750.042.3349534
AACGCAG152350.041.7387356
CAACGCA154850.041.4106065
ATCAACG155650.041.2753563
TATCAAC174250.037.074842
ACGCAGA181500.035.099817
CGCAGAG188200.033.945068
GCAGAGT203550.031.5021049
GTACATG185000.029.5737441
TACATGG189450.028.448272
ACATGGG189600.027.8606243
AGAGTAC192050.026.17184410-11
CATGGGG97750.025.7411354
CAGAGTA198550.024.80574810-11
GAGTACT89450.024.77067812-13
AGTACTT93450.023.45579112-13
CATGGGT52450.023.2494354
GTACTTT104750.021.12422814-15
Produced by FastQC (version 0.11.5)