FastQCFastQC Report
Fri 10 Feb 2017
ERR1147551.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147551.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4062926
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200690.49395435703234564No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116370.28641919641165015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90420.22254897086483977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71350.059.577741
GTATCAA147200.059.1720051
TCAACGC197300.043.9012834
AACGCAG198900.043.4584056
ATCAACG199300.043.40373
CAACGCA202300.042.757425
TATCAAC216800.040.0099452
ACGCAGA230550.037.490577
CGCAGAG237600.036.3021558
GCAGAGT256550.033.736649
GTACATG234650.032.045171
TACATGG242350.030.7593922
ACATGGG244300.030.1729343
AGAGTAC243150.027.35132810-11
CATGGGG128900.026.9065824
CATGGGT68250.026.759544
GAGTACT109500.026.48325512-13
CAGAGTA251650.025.7774310-11
ATGGGGG59600.025.2532465
AGTACTT116200.024.85386712-13
Produced by FastQC (version 0.11.5)