FastQCFastQC Report
Fri 10 Feb 2017
ERR1147550.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147550.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5644321
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249840.44263960182278794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140170.2483381083393379No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118900.21065421332344494No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA189000.057.1702881
GGTATCA95000.056.712731
TCAACGC248900.043.3046724
AACGCAG252500.042.592656
ATCAACG255200.042.1675723
CAACGCA256100.041.9939235
TATCAAC278500.039.0462572
ACGCAGA294900.036.5484967
CGCAGAG304550.035.449018
GTACATG315850.032.9847151
GCAGAGT335000.032.36899
TACATGG327150.031.8759692
ACATGGG327000.031.417043
CATGGGG172450.028.526194
AGAGTAC316250.027.1407510-11
CATGGGT93800.026.9517864
ATGGGGG84100.025.321225
CAGAGTA328100.024.6739110-11
AGTACTT154250.024.46766512-13
GAGTACT145150.024.36244612-13
Produced by FastQC (version 0.11.5)