FastQCFastQC Report
Fri 10 Feb 2017
ERR1147549.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147549.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5607144
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225700.4025222109508869No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122700.21882798087582556No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103870.18524582211550122No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA194650.055.622421
GGTATCA101500.049.3175541
AACGCAG249150.043.11446
TCAACGC252700.042.6499444
CAACGCA257550.041.6620335
ATCAACG259150.041.5902823
TATCAAC284700.038.192112
ACGCAGA292500.036.7239727
CGCAGAG302950.035.5157938
GCAGAGT332300.032.5041969
GTACATG310450.029.7174551
TACATGG323400.028.3798942
ACATGGG320100.028.2450773
CATGGGT96750.027.5418154
AGAGTAC311600.027.25741410-11
GAGTACT130900.024.67255212-13
CAGAGTA320950.024.55457310-11
AGTACTT138700.024.12125812-13
GTATAGG13850.024.0663281
CATGGGG157350.024.0033844
Produced by FastQC (version 0.11.5)