FastQCFastQC Report
Fri 10 Feb 2017
ERR1147548.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147548.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3611014
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201910.5591504214605648No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106630.29529101797999124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95760.26518866999684854No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA143450.056.3923231
GGTATCA73800.046.781341
AACGCAG181750.044.347096
TCAACGC189100.042.7177664
ATCAACG190600.042.4445843
CAACGCA192550.041.8905875
TATCAAC203950.039.9876632
ACGCAGA214600.037.585847
CGCAGAG220050.036.6544388
GCAGAGT239200.033.8442739
GTACATG214850.033.5236321
TACATGG220350.032.719142
ACATGGG220500.032.399683
CATGGGG118350.029.7545744
CATGGGT53400.029.7420084
AGAGTAC230050.027.73085810-11
ATGGGGG55800.026.0109315
AGTACTT122850.025.29876912-13
GAGTACT117800.024.69174812-13
CAGAGTA236600.024.33598110-11
Produced by FastQC (version 0.11.5)