FastQCFastQC Report
Fri 10 Feb 2017
ERR1147547.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147547.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3103728
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186880.6021146182912935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111770.3601153193836573No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94320.3038926091461623No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33280.10722589092858653No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32670.10526051251913827No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA140800.053.2321741
GGTATCA77650.048.8370131
AACGCAG179450.041.3389246
ATCAACG185350.040.1848073
TCAACGC185650.040.1506234
CAACGCA190250.039.0860255
TATCAAC198300.037.770542
ACGCAGA212050.035.0105137
CGCAGAG215850.034.449288
TATCACG1902.910383E-1134.442072
GTACATG215700.032.2913971
GCAGAGT234100.031.839919
TACATGG219650.031.7970662
ATGGGGG65500.030.8797515
ACATGGG221250.030.8142433
CATGGGG118300.030.4736214
CATGGGT53500.028.2433414
AGAGTAC225500.026.44600110-11
GAGTACT105100.026.23428312-13
TGGGGGG57100.025.8375766
Produced by FastQC (version 0.11.5)