FastQCFastQC Report
Fri 10 Feb 2017
ERR1147546.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147546.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316815
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151130.6523179451099894No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89210.38505448212308707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71710.3095197501742694No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24110.10406527927348537No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60100.053.7797471
GTATCAA107650.051.5894621
AACGCAG139850.039.3443156
TCAACGC142900.038.6710744
ATCAACG144000.038.3351863
TATCAAC145150.038.0314642
CAACGCA146900.037.5370985
ACGCAGA161550.034.058717
CGCAGAG165800.033.2215468
GCAGAGT179750.030.7094789
GTACATG145500.030.1916451
TACATGG151800.028.8807072
ACATGGG151450.028.476123
CATGGGG79600.027.126664
AGAGTAC172350.026.00550810-11
GAGTACT91800.024.8170112-13
CAGAGTA177200.024.70628510-11
AGTACTT95950.024.6735412-13
ATGGGGG37500.023.4764925
CATGGGT30500.022.4285264
Produced by FastQC (version 0.11.5)