FastQCFastQC Report
Fri 10 Feb 2017
ERR1147545.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147545.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375428
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84820.6166807713671671No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56450.4104177027078117No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38280.27831336863870737No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40450.058.2627751
GTATCAA71200.050.402471
AACGCAG85950.041.9361576
TCAACGC92300.038.9221734
ATCAACG92800.038.584283
CAACGCA94300.037.9705285
TATCAAC97500.037.1513442
ACGCAGA100950.035.7049267
CGCAGAG103100.034.9603548
GCAGAGT110950.032.5404249
GTACATG93350.030.0276341
TACATGG94850.029.7864072
CATGGGT29000.029.5342754
ACATGGG95300.029.2088743
CAGAGTA111700.026.73082710-11
GAGTACT57050.026.53346612-13
AGCGTCA4550.026.1445063
AGAGTAC108700.025.7996610-11
GTAATAC2151.2366581E-724.8980833
CATGGGG45350.024.5259674
Produced by FastQC (version 0.11.5)