FastQCFastQC Report
Fri 10 Feb 2017
ERR1147544.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147544.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2704150
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241240.8921102749477655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143370.5301850858865077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118140.43688404859197905No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42120.15576059020394578No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31160.11523029417746797No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT29440.10886970027550247No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA176450.053.4669381
GGTATCA95750.049.54471
AACGCAG213200.043.888036
ATCAACG230250.040.715633
TCAACGC231150.040.5056344
CAACGCA235400.039.749065
TATCAAC237900.039.5563852
ACGCAGA249150.037.5785837
CGCAGAG251200.037.319278
GCAGAGT268450.035.0763249
GTACATG229850.031.1270281
TACATGG234700.030.540672
CATGGGT65600.030.1050154
ACATGGG236650.029.8365633
CATGGGG116550.028.3260044
AGAGTAC262450.027.8775810-11
GAGTACT128000.027.65053612-13
ATGGGGG48700.027.1161885
AGTACTT132250.027.09929312-13
CAGAGTA265850.026.63724310-11
Produced by FastQC (version 0.11.5)