FastQCFastQC Report
Fri 10 Feb 2017
ERR1147543.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147543.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3596507
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207760.577671613040097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115140.32014396190525973No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99560.2768241518784754No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA154450.055.4294431
GGTATCA81850.050.333481
AACGCAG200500.042.6642076
TCAACGC209550.040.8216324
ATCAACG211850.040.3526083
CAACGCA214400.039.7872165
TATCAAC224600.038.1948552
ACGCAGA235050.036.416287
CGCAGAG240900.035.5813338
GCAGAGT257150.033.4947749
GTACATG235850.032.8910751
TACATGG242550.032.0080032
ACATGGG243600.031.356753
CATGGGG129350.029.0189764
CATGGGT64050.028.5127054
AGAGTAC250000.028.02852810-11
ATGGGGG62650.027.0609465
TATAACG3350.026.6376442
AGTACTT122150.025.76067412-13
GAGTACT117550.025.12416612-13
Produced by FastQC (version 0.11.5)