FastQCFastQC Report
Fri 10 Feb 2017
ERR1147542.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147542.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3679016
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251170.6827097245567837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137460.3736325147811263No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115500.31394264118449067No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39640.10774620170175939No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA188450.062.0044671
GGTATCA87500.060.2732281
AACGCAG245000.047.124986
ATCAACG249350.046.42533
TCAACGC249800.046.339154
CAACGCA254650.045.3391725
TATCAAC259250.044.8819122
ACGCAGA283600.040.856097
CGCAGAG290200.039.967898
GCAGAGT310350.037.4879049
GTACATG291850.033.163451
TACATGG297950.032.304012
ATGGGGG78000.032.2580075
ACATGGG300950.031.5075933
CATGGGG152850.030.666094
CATGGGT92750.030.3991934
AGAGTAC301800.029.23764410-11
GAGTACT135700.027.81159212-13
TGGGGGG66150.027.6057956
CAGAGTA307400.027.55371510-11
Produced by FastQC (version 0.11.5)