FastQCFastQC Report
Fri 10 Feb 2017
ERR1147541.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147541.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2497630
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119290.4776127769125131No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71920.28795297942449444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58820.23550325708771913No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30180.12083455115449446No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT26190.10485940671756824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44100.057.9027631
GTATCAA83950.056.438171
TCAACGC111250.042.2362984
AACGCAG111550.042.1227076
ATCAACG113000.041.530393
CAACGCA114050.041.0950665
TATCAAC125950.037.6380922
ACGCAGA132300.035.381297
CGCAGAG136150.034.4244778
GTACATG133700.033.7011761
TACATGG137700.032.439452
GCAGAGT146000.032.183489
ACATGGG138550.031.7252733
CATGGGG75850.029.40594
AGAGTAC140000.027.42375810-11
TACCGTA1107.7703147E-427.0356047
ATGGGGG38300.026.2450085
CATGGGT37200.025.26234
AGTACTT71500.025.08072312-13
CAGAGTA144250.025.04893110-11
Produced by FastQC (version 0.11.5)