FastQCFastQC Report
Fri 10 Feb 2017
ERR1147540.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147540.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404208
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99190.41256829691940133No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61080.2540545576755422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47460.19740388518797045No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24620.10240378536299688No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA87400.049.9197771
GGTATCA50850.048.6947821
AACGCAG109950.039.710916
TCAACGC111600.039.2303924
ATCAACG112850.038.7431373
CAACGCA114400.038.1142165
TATCAAC123900.035.3358462
ACGCAGA129800.033.6373187
CGCAGAG135550.032.2536438
GTACATG135700.031.2744561
TACATGG140350.030.092272
GCAGAGT147150.029.9131689
ACATGGG141250.029.5215113
CATGGGG79000.028.4625454
ATGGGGG40550.026.9920585
TATCACG1807.311646E-726.4378722
TAACGCA2558.8584784E-1025.660294
AGAGTAC137650.024.869110-11
CAGAGTA143550.023.7640910-11
GAGTACT65700.023.5395912-13
Produced by FastQC (version 0.11.5)