FastQCFastQC Report
Fri 10 Feb 2017
ERR1147538.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147538.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4969921
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260910.5249781636368063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163910.32980403511444145No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126230.25398794065338265No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT71830.1445294603274378No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA60870.12247679590882833No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT59550.11982081807738997No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG59070.11885500795686692No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT58080.11686302458328815No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA55920.11251687904093445No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC55420.11151082683205629No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC53150.1069433498037494No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC53010.10666165518526351No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT51220.10305998827747967No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA50890.10239599381962007No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA50830.10227526755455468No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA49710.10002171060666759No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101900.064.4924551
GTATCAA197550.055.4440351
AACGCAG255100.042.4134946
TCAACGC264000.041.2314874
ATCAACG267150.040.7688183
CAACGCA268950.040.2514655
TATCAAC295600.036.9858862
ACGCAGA296550.036.4647677
CGCAGAG304200.035.58658
GCAGAGT322750.033.6148879
GTACATG287150.030.7844681
TACATGG295150.029.9079072
ACATGGG294800.029.6205793
CATGGGT94700.028.3275094
GAGTACT146050.027.10313812-13
CAGAGTA322000.027.0709210-11
AGAGTAC315350.027.0382110-11
CATGGGG149350.026.8831624
GTACTTT160150.024.67977114-15
AGTACTT151850.024.40317512-13
Produced by FastQC (version 0.11.5)