FastQCFastQC Report
Fri 10 Feb 2017
ERR1147537.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147537.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3750489
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264330.7047880956323295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146350.39021578252862493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125980.3359028649330794No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44330.11819792032452302No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38450.10251996472993255No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA186750.057.3438951
GGTATCA95100.051.8908351
AACGCAG233650.045.5476346
TCAACGC244750.043.627774
ATCAACG247300.043.1550033
CAACGCA249750.042.6590655
TATCAAC259750.041.362482
ACGCAGA273650.038.8453257
CGCAGAG280500.037.939118
GCAGAGT300000.035.552389
GTACATG267000.033.576111
TACATGG274550.032.350632
ACATGGG275100.031.7661113
CATGGGT81100.030.6603184
CATGGGG136250.028.9903074
AGAGTAC293450.027.95373210-11
ATGGGGG60350.027.402415
GAGTACT142400.026.67230812-13
CAGAGTA298050.026.25496310-11
AGTACTT149400.026.01984212-13
Produced by FastQC (version 0.11.5)