FastQCFastQC Report
Fri 10 Feb 2017
ERR1147535.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147535.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2685389
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154070.5737343826164478No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89600.33365743287099187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70050.2608560621943413No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA132900.055.2320481
GGTATCA70300.048.9469381
AACGCAG169300.043.0787356
TCAACGC177700.041.1093254
ATCAACG177900.041.098083
CAACGCA181250.040.271335
TATCAAC183400.040.0926482
ACGCAGA199300.036.533177
CGCAGAG204550.035.5935178
GCAGAGT217900.033.494729
GTACATG209850.031.5463281
TACATGG211600.031.1508352
ACATGGG211500.030.856163
CATGGGG110600.030.3894444
ATGGGGG51950.029.887145
CATGGGT57000.029.4309084
TATCACG1954.796857E-827.4570262
AGAGTAC210650.027.36221910-11
TGGGGGG44850.025.7317146
GAGTACT87200.025.54608312-13
Produced by FastQC (version 0.11.5)