FastQCFastQC Report
Fri 10 Feb 2017
ERR1147534.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147534.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3551208
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261090.7352146086627424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148460.4180549266615754No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119030.3351817184462301No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46410.13068792365865362No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA188450.055.6571241
GGTATCA98900.050.799231
AACGCAG238950.043.513836
ATCAACG249950.041.504233
TCAACGC251200.041.3681534
CAACGCA256150.040.42945
TATCAAC260500.040.0516932
ACGCAGA281700.036.9525267
CGCAGAG288050.036.1792148
GCAGAGT308350.033.855259
GTACATG270800.032.115341
TACATGG277100.031.3841132
ACATGGG275400.031.167463
CATGGGT83600.031.1645414
CATGGGG134450.029.111064
AGAGTAC299600.027.36893510-11
GAGTACT143400.026.17418312-13
ATGGGGG60450.025.7808885
AGTACTT148750.025.73264712-13
ATGGGTG45350.025.4458735
Produced by FastQC (version 0.11.5)