FastQCFastQC Report
Fri 10 Feb 2017
ERR1147533.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147533.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1976055
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104450.5285784049533034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66720.33764242392038685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53170.2690714580312795No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20390.10318538704641318No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA102400.045.4512371
GGTATCA61400.043.5228041
AACGCAG123200.037.466936
TCAACGC130600.035.3439944
ATCAACG132400.034.819443
CAACGCA133550.034.652365
TATCAAC137400.033.6822362
ACGCAGA143350.032.1588947
CGCAGAG145750.031.5877348
ATGGGGG38500.031.0550635
CATGGGG79100.030.4561944
GTACATG144050.030.4090921
TATCACG1203.7036792E-529.742542
TACATGG147350.029.6315252
ACATGGG145550.029.4666733
GCAGAGT158600.029.2159739
TATAACG1704.437079E-727.992982
TGGGGGG32700.027.6498576
AGAGTAC154650.024.75054410-11
AGTACTT73900.023.74453512-13
Produced by FastQC (version 0.11.5)