FastQCFastQC Report
Fri 10 Feb 2017
ERR1147532.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147532.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881999
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112910.5999471838189074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75880.40318831200229116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60080.31923502616101285No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21250.1129118559574155No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57650.046.976681
GTATCAA95750.046.3735471
AACGCAG111400.039.3517466
TCAACGC121050.036.2146534
ATCAACG121600.036.148693
CAACGCA122850.035.684045
ACGCAGA130800.033.3787467
TATCAAC132700.033.2154772
CGCAGAG133350.032.7841878
GCAGAGT143650.030.4749039
GTACATG118750.028.3194661
ACATGGG119450.027.7862343
TACATGG122700.027.390322
AGAGTAC138100.025.28218310-11
CAGAGTA141750.024.94588510-11
GAGTACT73950.024.08955612-13
CATGGGG57800.023.9778254
CATGGGT29100.023.5064224
ATGGGTA16000.022.677315
AGTACTT77400.022.51628912-13
Produced by FastQC (version 0.11.5)