FastQCFastQC Report
Fri 10 Feb 2017
ERR1147531.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147531.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3048926
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204640.6711871655789612No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125520.41168595105292816No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98570.323294169815863No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36870.12092782835660819No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT36400.11938630193058146No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA30510.10006802395335275No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA158050.049.8230821
GGTATCA89250.045.4821931
AACGCAG189700.041.325126
TATAACG1900.040.697042
ATCAACG207900.037.7938773
TCAACGC208850.037.6213464
CAACGCA213100.036.731485
TATCAAC224950.035.220162
ACGCAGA223400.035.1166737
CGCAGAG227850.034.456378
GCAGAGT242300.032.4505969
GTACATG203650.031.0096041
TACATGG207450.030.2777522
ACATGGG208500.029.8399933
CATGGGT58950.027.7469254
AGAGTAC235150.027.15199310-11
CATGGGG98450.026.7641834
TATCACG1807.3180126E-726.4356862
AGTACTT126900.025.52001212-13
ATGGGTG30600.024.8802385
Produced by FastQC (version 0.11.5)