FastQCFastQC Report
Fri 10 Feb 2017
ERR1147530.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147530.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3383693
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178260.5268208433802948No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104220.30800666608938815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84320.24919518407846103No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA134550.056.055431
GGTATCA68500.053.6190871
AACGCAG172000.043.5105446
TCAACGC178600.042.102514
ATCAACG181300.041.443913
CAACGCA182050.041.1739165
TATCAAC195350.038.7068022
ACGCAGA204450.036.6320887
CGCAGAG209700.035.7990238
GTACATG213700.034.290861
GCAGAGT228200.032.948969
ACATGGG218850.032.7019963
TACATGG221950.032.700912
CATGGGT63600.029.651354
CATGGGG112550.028.4366934
AGAGTAC216750.027.89706610-11
ATGGGGG52100.027.5183035
TATAACG3300.027.0416052
TATCACG2455.5661076E-1026.7104842
GAGTACT102300.025.46881512-13
Produced by FastQC (version 0.11.5)