FastQCFastQC Report
Fri 10 Feb 2017
ERR1147529.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147529.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3594879
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280340.7798315325773134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159000.4422958324883814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134040.3728637319920921No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42400.11794555533023504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA205600.059.5833851
GGTATCA100850.057.7842751
AACGCAG269450.045.2087336
ATCAACG273300.044.532063
TCAACGC275550.044.2085344
TATCAAC281800.043.505462
CAACGCA280700.043.3332675
ACGCAGA313050.038.893297
CGCAGAG317100.038.3777858
GCAGAGT336550.036.230549
GTACATG310150.033.4716871
CATGGGT123050.033.1120074
TACATGG315600.032.720472
ACATGGG315150.032.2575653
ATGGGTG61600.030.3193195
CATGGGG138800.028.7547534
ATGGGGG64150.028.372355
AGAGTAC332400.028.30844510-11
GAGTACT155300.026.96321912-13
CAGAGTA337150.026.79817210-11
Produced by FastQC (version 0.11.5)