FastQCFastQC Report
Fri 10 Feb 2017
ERR1147528.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147528.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3812429
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234820.6159327819613165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115820.3037958215090694No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109200.2864315637091209No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT38960.1021920670522651No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA170650.057.3861351
GGTATCA90350.046.518311
AACGCAG218200.044.5430566
ATCAACG226750.042.814393
TCAACGC227150.042.7891244
CAACGCA231850.041.894965
TATCAAC244500.040.1446462
ACGCAGA256750.037.785097
CGCAGAG262450.036.9639748
GCAGAGT285700.034.1432539
CATGGGT82500.033.094274
GTACATG259000.032.0413171
TACATGG263650.031.5203632
TATAACG3050.031.2063312
ACATGGG263000.030.91933
ATGGGTG44200.028.7987845
AGAGTAC275200.028.09740610-11
AGTACTT135100.026.26191312-13
CATGGGG128100.026.2357464
TATCACG2507.039489E-1026.1743092
Produced by FastQC (version 0.11.5)