FastQCFastQC Report
Fri 10 Feb 2017
ERR1147527.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147527.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4756657
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209930.44133936922506706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123590.2598253353142764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91750.19288756788643788No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59900.071.85741
GTATCAA136050.063.9310341
TCAACGC179550.047.675244
ATCAACG184900.046.363053
CAACGCA184700.046.184395
TATCAAC188000.045.9782752
AACGCAG191900.044.5755656
ACGCAGA228400.037.4773187
CGCAGAG237250.036.1545378
GCAGAGT262500.032.74489
GTACATG220050.028.2989541
AGAGTAC242400.027.10422110-11
TACATGG229650.026.7595482
ACATGGG230950.026.2998163
GAGTACT120150.025.32361612-13
CAGAGTA255500.025.19069710-11
CATGGGG146600.024.386984
AGTACTT130450.023.57492312-13
GTACTTT133350.022.81688914-15
ATGGGGG79850.020.8591235
Produced by FastQC (version 0.11.5)