FastQCFastQC Report
Fri 10 Feb 2017
ERR1147526.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147526.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2174466
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120320.5533312546620641No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75930.34918918024011414No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55450.2550051368933798No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48050.053.1364671
GTATCAA87800.051.787821
AACGCAG114050.039.5314756
TCAACGC114150.039.4447334
ATCAACG116300.038.7164233
CAACGCA116350.038.647775
TATCAAC119800.037.684612
ACGCAGA133400.033.663577
CGCAGAG137250.032.762618
GCAGAGT149250.030.4073989
GTACATG121600.027.653091
TACATGG126550.026.6031682
ACATGGG125450.026.2200533
AGAGTAC141050.025.97626710-11
GAGTACT73350.025.62453712-13
CAGAGTA145500.024.4050910-11
AGTACTT77200.024.15401512-13
CATGGGG65650.023.0127284
GTACTTT86350.021.83564414-15
TATACCG1703.9442434E-420.992855
Produced by FastQC (version 0.11.5)