FastQCFastQC Report
Fri 10 Feb 2017
ERR1147525.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147525.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4464005
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280060.627373849267642No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150730.33765643183643385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126240.2827953821736311No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46060.10318088801423834No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA193600.055.774981
GGTATCA101400.049.546381
AACGCAG247550.043.130356
TCAACGC255350.041.9535754
ATCAACG257000.041.6393363
CAACGCA261650.040.828845
TATCAAC272050.039.555362
ACGCAGA288400.036.979967
CGCAGAG295550.036.0450828
GCAGAGT315100.033.940859
GTACATG265200.030.4139791
TACATGG274050.029.4771462
ACATGGG275000.028.9204123
AGAGTAC303350.027.76519410-11
CATGGGG140650.026.7704184
AGTACTT159300.025.35335512-13
GAGTACT152550.025.2664512-13
CAGAGTA313550.025.05030610-11
CATGGGT72950.024.135574
AGTACAT201850.021.9095612-13
Produced by FastQC (version 0.11.5)