FastQCFastQC Report
Fri 10 Feb 2017
ERR1147524.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147524.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3319211
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184860.5569395859437679No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113020.34050260739675786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88200.265725800498974No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35180.10598904378179032No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71950.055.0236431
GTATCAA131450.052.6717341
AACGCAG174300.039.4876
TCAACGC175900.039.0940064
ATCAACG177900.038.6891023
CAACGCA180600.038.0436675
TATCAAC188350.036.6688962
ACGCAGA204450.033.6623767
CGCAGAG210100.032.7005048
GTACATG193850.031.048731
TACATGG197050.030.4611282
GCAGAGT226400.030.3987319
ACATGGG198650.029.7066943
CATGGGG112600.027.9998444
ATGGGGG58550.025.8062555
AGAGTAC216700.025.59696610-11
CATGGGT45150.025.1648164
GAGTACT112350.024.61945312-13
CAGAGTA222850.024.22325710-11
AGTACTT115350.023.25720212-13
Produced by FastQC (version 0.11.5)