FastQCFastQC Report
Fri 10 Feb 2017
ERR1147523.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147523.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3610891
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160660.4449317356851813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93350.2585234503063094No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74310.20579408240237657No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA128950.053.313751
GGTATCA68650.052.195681
TCAACGC165350.041.0842934
AACGCAG165750.040.985156
ATCAACG167600.040.5344283
CAACGCA169700.039.9961055
TATCAAC186650.036.7803422
ACGCAGA199750.033.97877
CGCAGAG207000.032.817368
GCAGAGT224100.030.41949
GTACATG206350.030.0855851
TACATGG210550.029.2430762
ACATGGG208250.028.965763
CATGGGG112000.026.3968624
ATGGGGG53900.026.2664955
AGAGTAC211700.025.27482810-11
CATGGGT57450.024.3327794
TAACGCA3700.024.11594
GAGTACT97950.024.01872412-13
TATCACG2502.0156222E-823.795672
Produced by FastQC (version 0.11.5)