FastQCFastQC Report
Fri 10 Feb 2017
ERR1147522.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147522.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4083236
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184230.45118626501137826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103800.2542101411723447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83660.20488651647859688No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA139700.058.546941
GGTATCA68750.056.539811
TCAACGC185200.043.6836784
ATCAACG187150.043.2301063
AACGCAG186650.043.2168356
CAACGCA189550.042.6184125
TATCAAC205900.039.7845842
ACGCAGA218950.036.8667537
CGCAGAG224750.035.968298
GCAGAGT244350.033.2535749
GTACATG227750.030.946191
TACATGG233050.030.2231542
ACATGGG233900.029.7572563
CATGGGT63500.027.260924
AGAGTAC231550.027.24368710-11
CATGGGG127150.026.6673454
GAGTACT103450.025.932612-13
CAGAGTA238700.025.20655310-11
AGTACTT108700.024.48857312-13
ATGGGTA34650.023.6917485
Produced by FastQC (version 0.11.5)