FastQCFastQC Report
Fri 10 Feb 2017
ERR1147521.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147521.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4392686
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299990.6829306715754324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173920.39593087236374286No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142500.3244028824277447No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54010.1229543837187543No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53440.12165677218904333No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC46200.10517482925025826No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97750.062.7289621
GTATCAA199950.059.933551
ATCAACG272900.043.7531743
TCAACGC273300.043.7306864
AACGCAG274250.043.470746
CAACGCA280100.042.5840765
TATCAAC288050.041.6796382
ACGCAGA325100.036.6513677
CGCAGAG330150.036.125968
GCAGAGT350350.034.144939
GTACATG323250.032.799941
ACATGGG329350.031.738023
TACATGG333150.031.7335242
CATGGGT104950.029.8159944
CATGGGG171550.029.3723474
ATGGGGG99500.028.1607255
AGAGTAC343550.027.66095710-11
GAGTACT155900.026.4997912-13
AGTACTT163650.026.26262512-13
TATCACG2757.4578566E-1125.9608692
Produced by FastQC (version 0.11.5)