FastQCFastQC Report
Fri 10 Feb 2017
ERR1147520.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147520.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4462518
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237070.5312471568742132No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124510.279012880172136No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110180.24690096488126212No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT45830.10269986586048505No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA193850.055.892091
GGTATCA100750.046.9750061
AACGCAG252400.042.6587226
TCAACGC257200.041.9319884
TATAACG3150.041.547542
ATCAACG260100.041.4882623
CAACGCA263350.040.8172235
TATCAAC272150.039.8921782
ACGCAGA298600.036.057677
CGCAGAG304000.035.436348
GCAGAGT324300.033.4382639
GTACATG318200.032.852551
TACATGG324300.031.9546762
ACATGGG326450.031.1789653
CATGGGG177350.030.2547784
ATGGGGG84600.030.0245255
CATGGGT82150.028.3131264
AGAGTAC316450.026.94614410-11
TGGGGGG69850.026.230286
AGTACTT142200.025.81009312-13
Produced by FastQC (version 0.11.5)