FastQCFastQC Report
Fri 10 Feb 2017
ERR1147519.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147519.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2548055
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148880.5842888006734549No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95540.3749526599700556No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74250.2913987335438207No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26150.1026272980763759No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25830.10137143821463822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48900.061.8531881
GTATCAA91750.054.2509651
AACGCAG121450.040.3594066
TCAACGC122500.040.159934
CAACGCA124400.039.4501465
ATCAACG125400.039.3276143
TATCAAC133250.037.323272
ACGCAGA143650.034.12157
CGCAGAG147800.033.3237538
GTACATG131950.031.99221
GCAGAGT159100.030.9943399
TACATGG136600.030.7464262
ACATGGG138900.029.5948683
CATGGGG79150.028.33444
AGAGTAC151800.026.60700610-11
CAGAGTA157650.025.28010210-11
GAGTACT81150.024.95896512-13
AGTACTT83300.024.74322312-13
ATGGGGG39000.024.7095855
TATAACG2102.7965998E-622.6626822
Produced by FastQC (version 0.11.5)