FastQCFastQC Report
Fri 10 Feb 2017
ERR1147517.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147517.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3145403
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155530.49446764055353165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94160.29935750681232265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75060.2386339683658978No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA125800.051.8130951
GGTATCA69000.049.1735841
AACGCAG154000.041.7928356
TCAACGC160950.040.0990454
ATCAACG163900.039.305353
CAACGCA164850.038.969975
TATCAAC170050.038.2336462
ACGCAGA180400.035.674547
CGCAGAG184950.034.79698
GCAGAGT202750.031.8006539
GTACATG174750.030.7593151
TACATGG181100.029.265872
ACATGGG178400.029.1086183
AGAGTAC192100.026.45771810-11
CATGGGG95650.025.6216754
CAGAGTA197150.024.93525110-11
GAGTACT97150.024.70406512-13
AGTACTT102900.023.72823512-13
CATGGGT40350.023.1446914
ATGGGGG45800.022.5984695
Produced by FastQC (version 0.11.5)