FastQCFastQC Report
Fri 10 Feb 2017
ERR1147516.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147516.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2737765
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131250.4794056465766784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79160.28914095987055133No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66140.24158391973014484No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA101450.052.9297561
GGTATCA54150.051.2311
AACGCAG126950.042.0719156
TCAACGC132250.040.47584
ATCAACG133550.040.0409243
CAACGCA134500.039.79875
TATCAAC145900.037.1408122
ACGCAGA151150.035.37537
CGCAGAG153500.034.8337258
GCAGAGT168000.031.9688579
GTACATG147600.029.8059841
TACATGG151800.028.8791242
ACATGGG152400.028.3751223
AGAGTAC157950.027.24379210-11
CATGGGG84500.027.1693484
ATGGGGG44150.026.1348275
GAGTACT74750.025.54126712-13
CAGAGTA165350.024.69363210-11
AGTACTT80150.024.41411612-13
GTAAGTA17900.023.9258692
Produced by FastQC (version 0.11.5)