FastQCFastQC Report
Fri 10 Feb 2017
ERR1147515.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147515.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3432219
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154690.45069967854615334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97560.28424759608871114No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74690.2176143188998138No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA120950.054.7282641
GGTATCA64300.054.7128451
AACGCAG154300.042.6754386
TCAACGC156500.042.037524
CAACGCA160000.041.006425
ATCAACG161350.040.739433
TATCAAC175450.037.702752
ACGCAGA181700.036.1408047
CGCAGAG187150.035.120138
GCAGAGT202800.032.439259
GTACATG182650.030.8959431
TACATGG189200.029.9006752
ACATGGG194700.028.5060673
CATGGGG100600.026.903564
AGAGTAC194050.026.57596810-11
GAGTACT94650.025.32608612-13
CAGAGTA199800.025.21573310-11
AGTACTT99750.023.61380212-13
ATGGGGG45600.023.480325
CATGGGT47600.022.6187154
Produced by FastQC (version 0.11.5)