FastQCFastQC Report
Fri 10 Feb 2017
ERR1147514.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147514.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4324657
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190170.4397342956909646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107480.24852838040103525No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87250.20175010411230299No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA144150.053.5190731
GGTATCA76150.053.3129431
TCAACGC187850.040.7850844
AACGCAG188600.040.591366
ATCAACG193100.039.6463363
CAACGCA192800.039.64545
TATCAAC203950.037.800112
ACGCAGA224550.034.1449557
CGCAGAG230750.033.252918
GCAGAGT253050.030.5810689
GTACATG221450.030.429091
TACATGG232200.028.9486312
ACATGGG231050.028.4487443
AGAGTAC238800.025.90482710-11
CATGGGG122850.025.7592494
CAGAGTA249950.023.64266410-11
AGTACTT128750.023.49229212-13
GAGTACT119050.023.30794112-13
ATGGGGG56900.022.2671225
CATGGGA91550.020.0119364
Produced by FastQC (version 0.11.5)