FastQCFastQC Report
Fri 10 Feb 2017
ERR1147513.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147513.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2271840
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114200.5026762448059723No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77290.34020881752236076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53580.23584407352630468No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53950.052.843241
GTATCAA91450.046.9242021
AACGCAG110950.038.0149086
TCAACGC115500.036.826384
CAACGCA117900.035.925375
ATCAACG118450.035.8087773
TATCAAC122650.034.825062
ACGCAGA134500.031.4465337
CGCAGAG138450.030.5923258
GTACATG124400.029.1846731
GCAGAGT149700.028.372789
TACATGG130500.027.7158452
ACATGGG130100.027.480983
CATGGGG71300.026.2818574
GAGTACT72050.024.81052612-13
TATAACG1701.4015919E-524.4953782
CAGAGTA145700.024.33392510-11
AGAGTAC141750.023.94186810-11
ATGGGGG32450.022.9155525
AGTACTT77100.022.45246712-13
Produced by FastQC (version 0.11.5)